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Learn how to protect yourself, your experiment, and work environment through these important tips for experimenting in a Class II biological safety cabinet (BSC). 

Maximize potential protection by ensuring you know how filtered and contaminated air from countertops and rooms pass through your cabinet. 

2. Work at the proper belt level

Your BSC will be certified for safe operation at a certain sash height-most cabinets will indicate where the windows should be placed during work activities. It is essential to work at a certified sash height to ensure that a balance between inflow and downward flow rates is maintained. Excessive inflow will cause contaminated air to enter the work area; too much downward flow will cause the contaminated air to enter the laboratory and generate turbulent air in the work area, resulting in product contamination. 

3. Do not block the airflow grill

The airflow grille at the front of the cabinet performs important work to separate the clean air inside the cabinet from the contaminated laboratory air outside. Be careful not to cover the air grille at the front of the cabinet with equipment, or even your arms, elbows or hands, as this will damage the integrity of the airflow, causing contaminants to enter the sterile work area in the cabinet, or material leaks from the cabinet inside. Go to the laboratory. 

The best way to work in the BSC is to slowly move your arms in and out, and perpendicular to the surface of the cabinet opening to minimize the interruption of airflow in the cabinet. It is also important to organize the cabinet work area so that minimal arm movement is required; and make sure to be careful when approaching the cabinet or opening and closing doors-rapid movement near the BSC may damage the cabinet's air barrier.

Use good microbiological techniques to reduce the potential risk of splashing or aerosols. The level II cabinet is designed so that the horizontally atomized spores will be captured within 14 inches of the cabinet air flowing downward. As a general rule of thumb, the cleaning material should be kept at least 12 inches away from the aerosol-generating activity to prevent cross-contamination.

The middle third of the BSC workbench is the ideal area for your experiment. All operations should be performed at least 4 inches from the front grille. The materials placed in the cabinet may cause interruptions in air flow, leading to turbulence, possible cross-contamination and/or leakage. Therefore, it is important to place only the materials needed for immediate work in the cabinet work area, and these materials should be placed as far back as possible, but still accessible. Large items such as biohazard bags should be placed on one side of the cabinet, and equipment that generates aerosols (such as vortex mixers or centrifuges) should be placed at the back of the cabinet. 

7. Work from clean to polluted

Active work should flow from the clean area to the contaminated area of ​​the entire work surface to limit the movement of contaminated equipment on clean items. 

The opened test tube and bottle should not be placed vertically, and the bottle cap should not be placed on the towel. The cap should be put back on the test tube or bottle as soon as possible. 

Then use the petri dish, the lid should be kept above the opened sterile surface to minimize the direct influence of downward air.

10. Use suction bottle or suction bottle 

The breathing bottle or suction bottle should be connected to an overflow collection bottle containing an appropriate disinfectant and an online HEPA or equivalent filter. 

Appropriate clothing is also essential for your personal protection as well as your product and work environment. Laboratory work clothes should be buttoned up and worn on street clothes, protective glasses should always be worn, and latex or nitrile gloves should be used when handling cultures, contaminated surfaces or equipment. 

Optimize your settings to improve your security. For example, use a high-quality laboratory chair and sit at a 90-degree seat and knee angle to reduce your risk of injury in the workplace. Also make sure that your wrists are straight and your work area is properly arranged to avoid over-extension of your arms-accessories such as armrests and footrests can be used to increase comfort and support. 

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Sarah Thomas Deputy Editor-in-Chief     

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